Atividade de promoção de crescimento de plantas de feijão de bactérias endofíticas isoladas de Echeveria laui
DOI:
https://doi.org/10.22571/2526-4338496Palavras-chave:
Erwinia sp.,, planta ornamental, Pantoea sp., Phaseolus vulgaris, endofitasResumo
Echeveria laui (Crassulaceae) é comumente comercializada devido à sua capacidade de tolerância à seca e à sua estética em forma de roseta. Uma vez que endófitos associados a plantas de ambiente seco ou árido foram poucos explorados até então, este trabalho compreende o isolamento de bactérias endofíticas de folhas de E. laui (uma planta de cinco anos e uma de dois anos) investigando a capacidade de agente promotor do crescimento de plantas das bactérias endofíticas isoladas como solubilizar fosfato, fixar nitrogênio, produzir exopolissacarídeos/ AIA e antagonizar fitopatógenos. O isolamento pela metodologia de maceração proporcionou uma taxa de colonização de 1.98 x109 UFC g-1 para a planta de dois anos e 1.14 x 1010 UFC g-1 para a de cinco anos. Todos os 40 isolados avaliados mostraram habilidades do agente promotor de crescimento de plantas in vitro, com ênfase em EG04, ELG18 e ELP06. A capacidade dos três melhores isolados bacterianos foi avaliada em casa de vegetação em plantas de feijão preto e comum (Phaseolus vulgaris L.). Com base no sequenciamento da região 16S rRNA e análise filogenética, os três endófitos foram identificados como Pantoea sp. (ELG04 e ELG18) e Erwinia sp. (ELP06). Em casa de vegetação, diferenças estatisticamente significativas foram encontradas entre as plantas tratadas com os três endófitos quando comparadas às plantas controle para o peso fresco e seco da parte aérea e raízes, bem como para o comprimento da raiz.
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Referências
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Bezerra, J.D., Azevedo, J.L., & Souza-Motta, M.C. (2017). Why study endophytic fungal comunity associated with Cacti species?. In: Azevedo, J.L., & Quecine, M.C. (Eds). Diversity and benefits of microorganisms from the tropics. Springer, p.21-36. doi: 10.1007/978-3-319-55804-2_2.
Breda, F.A.F., Alves, G.C., & Reis, V.M. (2016). Productivity of mize in the presence of nitrogen levels and inoculation with Herbaspirillum seropedice. Pesquisa Agropecuária Brasileira, 51(1), 45-52. doi: 10.1590/S0100-204X2016000100006.
Chen, C., Xin, K., Liu, H., Cheng, J., Shen, X., Wang, Y., & Zhang, L. (2017). Pantoea alhagi, a novel endophytic bacterium with ability to improve growth and drought tolerance in wheat. Scientific Reports, 7,41564. doi: 10.1038/srep41564.
Collavino, M.M., Sansberro, P.A., Mroginski, L.A., & Aguilar, O.M. (2010). Comparison of in vitro solubilization activity of diverse phosphate-solubilizing bacteria native to acid soil and their ability to promote Phaseolus vulgaris growth. Biology and Fertility Soils, 46, 727-738. doi: doi: 10.1007/s00374-010-0480-x.
Comby, M., Gacoin, M., Robineau, M., Rabenoelina, F., Ptas, S.,Dupont, J., Profizi, C., & Baillieu, F. (2017). Screening of wheat endophytes as biological control agents against Fusarium head blight using two different in vitro tests. Microbiological Research, 202, 11-20. doi: 10.1016/j.micres.2017.04.014.
Godeau, G., Laugier, J.P., Orange, F., Godeau, R.P., Guittard, F., & Darmanin, T. (2017). A travel in the Echeveria genus wettability's world. Applied Surface Science, 411, 291-302. doi: doi: 10.1016/j.apsusc.2017.03.192.
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Kavamura, V.N., Santos, S.N., Silva, J.L., Parma, M.M., Ávilla, L.A., Visconti, A., Zucchi, T.D., Taketani, R.G., Andreote, F.D., & Melo, I.S. (2013). Screening of Brazilian cacti rhizobacteria for plant growth promotion under drought. Microbiological Research, 168(4), 183-191. doi: 10.1016/j.micres.2012.12.002.
López-Ângulo, G., Montes-Avila, J., Díaz-Camacho, S.P., Veja-Aviña, R., Báez-Flores, M.H, & Delgardo-Vargas, F. (2016). Bioactive components and antimutagenic and antioxidant activities of two Echeveria DC. species. Industrial Crops and Products, 85, 38-48. doi: 10.1016/j.indcrop.2016.02.044.
López-Gonzáles, R.C., Gómez-Cornelio, S., La Rosa-García, S.C., Garrido, E., Oropeza-Mariano, O., Heil, M., & Partida-Martínez, L.P. (2017). The age of lima bean leaves influences the richness and diversity of the endophytic fungal community, but not the antagonistic effect of endophytes against. Colletotrichum lindemuthianum. Fungal Ecology, 26, 1–10. doi: 10.1016/j.funeco.2016.11.004.
Ma, Y., Oliveira, R.S., Nai, F., Rajkamar, M., Luo, Y., Rocha, I., & Freitas, H. (2015). The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil. Journal of Environmental Management, 156, 62-69. doi: 10.1016/j.jenvman.2015.03.024.
Mishra, A., Chauhan, P.S., Chaudhry, V., Tripathi, M., & Nautiyal, C.S. (2011). Rhizosphere competent Pantoea agglomerans enhances maize (Zea mays) and chickpea (Cicer arietinum L.) growth, without altering the rhizosphere functional diversity. Antonie van Leeuwenhoek, 100, 405–413. doi: 10.1007/s10482-011-9596-8.
Neetha, J.N., Sandesh, K., Kumar, K.G.., Chidamamda, B., & Ujwal, P. (2019). Optimization of Direct Blue-14 dye degradation by Bacillus fermus (Kx898362) an alkaliphilic plant endophyte and assessment of degraded metabolite toxicity. Journal of Hazardous Materials, 364, 742-751. doi: 10.1016/j.jhazmat.2018.10.074.
Niknezhad, S.V., Morowvat, M.H., Darz, G.N., Iraji, A., & Ghasemi, Y. (2018). Exopolysaccharide from Pantoea sp. BCCS 001 GH isolated from nectarine fruit: production in submerged culture and preliminary physicochemical characterizations. Food Science and Biotechnology, 27(6), 1735–1746. doi: 10.1007/s10068-018-0409-y.
Oliveira, J.A., Polli, A.D., Polonio, J.C., Orlandelli, R.C., Conte, H., Azevedo, J.L., & Pamphile, J.A. (2020). Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit. Acta Scientiarum Biological Sciences, 42, 1-11. doi: 10.4025/actascibiolsci.v42i1.48321.
Sánchez-Cruz, R., Vázquez, I.T., Batista-Garcia, R.A., Méndez-Santiago, E.W., Sánchez-Carbente, M.R., Leija, A., Lira-Ruan, V. Hernández, G., Wong-Villarreal, A., & Folch-Mallol, L. (2019). Isolation and characterization of endophytes from nodules of Mimosa pudica with biotechnological potential. Microbiological Research, 218, 76-86. doi: 10.1016/j.micres.2018.09.008.
Sergeeva, E., Hirkala, D.L.M., & Nelson, L.M. (2007). Production of indole-3-acetic acid, aromatic amino acid aminotransferase activities and plant growth promotion by Pantoea agglomerans rhizosphere isolates. Plant Soil, 297, 1-13. doi: 10.1007/s11104-007-9314-5.
Silini-Chérif, H., Silini, A., Ghoul, M., & Yadav, S. (2012). Isolation and characterization of plant growth promoting traits of a rhizobacteria: Pantoea agglomerans lma2. Pakistan Journal of Biological Sciences, 15(6), 267-276. doi: 10.3923/pjbs.2012.267.276.
Stevens, J.F., Hart, H., Ham, R.C.H.J., Elema, E.T, Van Den Ent, M.M.V.X., Wildeboer, M., & Zwaving, J.H. (1995). Distribution of Alkaloids and Tannins in the Crassulaceae. Biochemical Systematics and Ecology, 23(2), 157-165. doi: 10.1016/0305-1978(95)00082-6.
Walterson, A.M., & Stavrinides, J. (2015). Pantoea: insights into a highly versatile and diverse genus within the Enterobacteriaceae. FEMS Microbiology Reviews, 39(6), 968-984. doi: 10.1093/femsre/fuv027.
Adhikari, P., & Pandey, A. (2019). Phosphate solubilization potential of endophytic fungi isolated from Taxus wallichiana Zucc. roots. Rhizosphere, 9, 02-09. doi: 10.1016/j.rhisph.2018.11.002.
Bezerra, J.D., Azevedo, J.L., & Souza-Motta, M.C. (2017). Why study endophytic fungal comunity associated with Cacti species?. In: Azevedo, J.L., & Quecine, M.C. (Eds). Diversity and benefits of microorganisms from the tropics. Springer, p.21-36. doi: 10.1007/978-3-319-55804-2_2.
Breda, F.A.F., Alves, G.C., & Reis, V.M. (2016). Productivity of mize in the presence of nitrogen levels and inoculation with Herbaspirillum seropedice. Pesquisa Agropecuária Brasileira, 51(1), 45-52. doi: 10.1590/S0100-204X2016000100006.
Chen, C., Xin, K., Liu, H., Cheng, J., Shen, X., Wang, Y., & Zhang, L. (2017). Pantoea alhagi, a novel endophytic bacterium with ability to improve growth and drought tolerance in wheat. Scientific Reports, 7,41564. doi: 10.1038/srep41564.
Collavino, M.M., Sansberro, P.A., Mroginski, L.A., & Aguilar, O.M. (2010). Comparison of in vitro solubilization activity of diverse phosphate-solubilizing bacteria native to acid soil and their ability to promote Phaseolus vulgaris growth. Biology and Fertility Soils, 46, 727-738. doi: doi: 10.1007/s00374-010-0480-x.
Comby, M., Gacoin, M., Robineau, M., Rabenoelina, F., Ptas, S.,Dupont, J., Profizi, C., & Baillieu, F. (2017). Screening of wheat endophytes as biological control agents against Fusarium head blight using two different in vitro tests. Microbiological Research, 202, 11-20. doi: 10.1016/j.micres.2017.04.014.
Godeau, G., Laugier, J.P., Orange, F., Godeau, R.P., Guittard, F., & Darmanin, T. (2017). A travel in the Echeveria genus wettability's world. Applied Surface Science, 411, 291-302. doi: doi: 10.1016/j.apsusc.2017.03.192.
Guo, L.D., Huang, G.R., & Wang, Y. (2008). Seasonal and tissue age influences on endophytic fungi of Pinus tabulaeformis (Pinaceae) in the Dongling Mountains, Beijing. Journal of Integrative Plant Biology, 50(8), 997-1003. doi: 10.1111/j.1744-7909.2008.00394.x.
Herrera, S.D., Grossi, C., Zawoznik, M., & Groppa, M.D. (2016). Wheat seeds harbour bacterial endophytes with potential as plantgrowth promoters and biocontrol agents of Fusarium graminearum. Microbiological Research, 187, 37-43. doi: 10.1016/j.micres.2016.03.002.
Jo, H., Jang, M., Hong, J.K., & Parque, C.J. (2018). First report of fungal leaf spot in Echeveria spp. caused by Cladosporium tenuissimum in Korea. Plant Desease, 102 (7), 1450. doi: 10.1094/PDIS-08-17-1277-PDN.
Kavamura, V.N., Santos, S.N., Silva, J.L., Parma, M.M., Ávilla, L.A., Visconti, A., Zucchi, T.D., Taketani, R.G., Andreote, F.D., & Melo, I.S. (2013). Screening of Brazilian cacti rhizobacteria for plant growth promotion under drought. Microbiological Research, 168(4), 183-191. doi: 10.1016/j.micres.2012.12.002.
López-Ângulo, G., Montes-Avila, J., Díaz-Camacho, S.P., Veja-Aviña, R., Báez-Flores, M.H, & Delgardo-Vargas, F. (2016). Bioactive components and antimutagenic and antioxidant activities of two Echeveria DC. species. Industrial Crops and Products, 85, 38-48. doi: 10.1016/j.indcrop.2016.02.044.
López-Gonzáles, R.C., Gómez-Cornelio, S., La Rosa-García, S.C., Garrido, E., Oropeza-Mariano, O., Heil, M., & Partida-Martínez, L.P. (2017). The age of lima bean leaves influences the richness and diversity of the endophytic fungal community, but not the antagonistic effect of endophytes against. Colletotrichum lindemuthianum. Fungal Ecology, 26, 1–10. doi: 10.1016/j.funeco.2016.11.004.
Ma, Y., Oliveira, R.S., Nai, F., Rajkamar, M., Luo, Y., Rocha, I., & Freitas, H. (2015). The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil. Journal of Environmental Management, 156, 62-69. doi: 10.1016/j.jenvman.2015.03.024.
Mishra, A., Chauhan, P.S., Chaudhry, V., Tripathi, M., & Nautiyal, C.S. (2011). Rhizosphere competent Pantoea agglomerans enhances maize (Zea mays) and chickpea (Cicer arietinum L.) growth, without altering the rhizosphere functional diversity. Antonie van Leeuwenhoek, 100, 405–413. doi: 10.1007/s10482-011-9596-8.
Neetha, J.N., Sandesh, K., Kumar, K.G.., Chidamamda, B., & Ujwal, P. (2019). Optimization of Direct Blue-14 dye degradation by Bacillus fermus (Kx898362) an alkaliphilic plant endophyte and assessment of degraded metabolite toxicity. Journal of Hazardous Materials, 364, 742-751. doi: 10.1016/j.jhazmat.2018.10.074.
Niknezhad, S.V., Morowvat, M.H., Darz, G.N., Iraji, A., & Ghasemi, Y. (2018). Exopolysaccharide from Pantoea sp. BCCS 001 GH isolated from nectarine fruit: production in submerged culture and preliminary physicochemical characterizations. Food Science and Biotechnology, 27(6), 1735–1746. doi: 10.1007/s10068-018-0409-y.
Oliveira, J.A., Polli, A.D., Polonio, J.C., Orlandelli, R.C., Conte, H., Azevedo, J.L., & Pamphile, J.A. (2020). Bioprospection and molecular phylogeny of culturable endophytic fungi associated with yellow passion fruit. Acta Scientiarum Biological Sciences, 42, 1-11. doi: 10.4025/actascibiolsci.v42i1.48321.
Sánchez-Cruz, R., Vázquez, I.T., Batista-Garcia, R.A., Méndez-Santiago, E.W., Sánchez-Carbente, M.R., Leija, A., Lira-Ruan, V. Hernández, G., Wong-Villarreal, A., & Folch-Mallol, L. (2019). Isolation and characterization of endophytes from nodules of Mimosa pudica with biotechnological potential. Microbiological Research, 218, 76-86. doi: 10.1016/j.micres.2018.09.008.
Sergeeva, E., Hirkala, D.L.M., & Nelson, L.M. (2007). Production of indole-3-acetic acid, aromatic amino acid aminotransferase activities and plant growth promotion by Pantoea agglomerans rhizosphere isolates. Plant Soil, 297, 1-13. doi: 10.1007/s11104-007-9314-5.
Silini-Chérif, H., Silini, A., Ghoul, M., & Yadav, S. (2012). Isolation and characterization of plant growth promoting traits of a rhizobacteria: Pantoea agglomerans lma2. Pakistan Journal of Biological Sciences, 15(6), 267-276. doi: 10.3923/pjbs.2012.267.276.
Stevens, J.F., Hart, H., Ham, R.C.H.J., Elema, E.T, Van Den Ent, M.M.V.X., Wildeboer, M., & Zwaving, J.H. (1995). Distribution of Alkaloids and Tannins in the Crassulaceae. Biochemical Systematics and Ecology, 23(2), 157-165. doi: 10.1016/0305-1978(95)00082-6.
Walterson, A.M., & Stavrinides, J. (2015). Pantoea: insights into a highly versatile and diverse genus within the Enterobacteriaceae. FEMS Microbiology Reviews, 39(6), 968-984. doi: 10.1093/femsre/fuv027.
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Publicado
2021-05-27
Edição
Seção
Microbiologia
