Controle do Aspergillus flavus em grãos de trigo através do óleo essencial de Cymbopogon flexuosus
DOI:
https://doi.org/10.22571/2526-4338528Palavras-chave:
Citral, CIM, Fungos, Capim limãoResumo
O fungo Aspergillus flavus Link (1809) é um dos principais contaminantes de grãos e é conhecido por produzir a aflatoxina. O método para conter fungos e outras pragas na agricultura é com o uso de agrotóxicos, porém estes causam danos a outras espécies, ao meio ambiente e a saúde humana, além das pragas adquirirem resistências a essas substâncias. Tem-se buscado alternativas naturais para controlar esses organismos. Nesse contexto, os óleos essenciais se apresentam como opção. Este estudo identificou os principais componentes e avaliou a eficácia do óleo essencial de Cymbopogon flexuosus Stapf (capim limão) para controle do fungo A. flavus. O efeito do óleo essencial sobre o crescimento micelial do fungo foi avaliado em testes in vitro nas doses entre 0,05 e 12,8 µL mL-1. O teste in vivo foi realizado nas seguintes concentrações: 0,6; 0,8 e 1,6 µL mL-1. O óleo essencial, apresentou composição rica em citral (formado pelos isômeros geranial e neral). A concentração inibitória mínima (CIM) foi de 0,8 μL mL-1, apresentou potencial fungicida sobre o A. flavus, demonstrando ser promissor na substituição de agrotóxicos, sendo uma alternativa de baixo impacto para a saúde e o meio ambiente.
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Referências
Achar, P. N., Quyen, P., Adukwu, E. C., Sharma, A., Msimanga, H. Z., Nagaraja, H., & Sreenivasa, M. Y. (2020). Investigation of the Antifungal and Anti-Aflatoxigenic Potential of Plant-Based Essential Oils against Aspergillus flavus in Peanuts. Journal of Fungi, 6(4), 383. doi: 10.3390/jof6040383
Adams, R. P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry (Vol. 456). Carol Stream, IL: Allured publishing corporation.
Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils–a review. Food and chemical toxicology, 46(2), 446-475. doi: 10.1016/j.fct.2007.09.106
Chao, S. C., Young, D. G., & Oberg, C. J. (2000). Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of essential oil research, 12(5), 639-649. doi: 10.1080/10412905.2000.9712177
Choi, H. S. (2003). Character impact odorants of Citrus Hallabong [(C. unshiu Marcov× C. sinensis Osbeck)× C. reticulata Blanco] cold-pressed peel oil. Journal of agricultural and food chemistry, 51(9), 2687-2692. doi: 10.1021/jf021069o
Billerbeck, V. G., Roques, C. G., Bessière, J. M., Fonvieille, J. L., & Dargent, R. (2001). Effects of Cymbopogon nardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger. Canadian journal of microbiology, 47(1), 9-17. doi: 10.1139/w00-117
Morais, L. A. S., Ramos, N. P., Gonçalves, G. G., Bettiol, W., & Chaves, F. C. M. (2008). Atividade antifúngica de óleos essenciais em sementes de feijão cv. Carioquinha. In Embrapa Amazônia Ocidental-Artigo em anais de congresso (ALICE). In: Congresso Brasileiro de Olericultura, 48., 2008, Maringá. Resumos... Maringá: Associação Brasileira de Horticultura, 2008. p. S6261-S6266
Dellavalle, P. D., Cabrera, A., Alem, D., Larrañaga, P., Ferreira, F., & Rizza, M. D. (2011). Antifungal activity of medicinal plant extracts against phytopathogenic fungus. Alternaria spp. Chilean journal of agricultural research, 71(2), 231-239. doi: 10.4067/S0718-58392011000200008
Ferreira, D. F. (2014). Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ciência e agrotecnologia, 38(2), 109-112. doi: 10.1590/S1413-70542014000200001.
Han, X., & Parker, T. L. (2017). Lemongrass (Cymbopogon flexuosus) essential oil demonstrated anti-inflammatory effect in pre-inflamed human dermal fibroblasts. Biochimie open, 4, 107-111. doi: 10.1016/j.biopen.2017.03.004
Lanças, F. M. (2009). A Cromatografia Líquida Moderna e a Espectrometria de Massas: finalmente “compatíveis”. Scientia chromatographica, 1(2), 35-61.
Lee, L. T., Garcia, S. A., Martinazzo, A. P., & Teodoro, C. E. S. (2020). Fungitoxity and chemical composition of rosemary essential oil (Rosmarinus officinalis) on Aspergillus flavus. Research, Society and Development, 9(8), e202985628. doi: 10.33448/rsd-v9i8.5628
Liu, Q., Li, X., Wu, R., Xiao, X., & Xing, F. (2021). Development of an on-spot and rapid recombinase polymerase amplification assay for Aspergillus flavus detection in grains. Food Control, 125, 107957 doi: 10.1016/j.foodcont.2021.107957
López-Malo, A., Alzamora, S. M., & Palou, E. (2002). Aspergillus flavus dose–response curves to selected natural and synthetic antimicrobials. International journal of food microbiology, 73(2-3), 213-218. doi: 10.1016/S0168-1605(01)00639-0
Lorenzi, H. (2008). Plantas daninhas do Brasil: terrestres, aquáticas, parasitas e tóxicas.
Luo, M., Jiang, L. K., Huang, Y. X., Xiao, M., Li, B., & Zou, G. L. (2004). Effects of citral on Aspergillus flavus spores by quasi-elastic light scattering and multiplex microanalysis techniques. Acta Biochimica et Biophysica Sinica, 36(4), 277-283. doi: 10.1093/abbs/36.4.277
Martinazzo, A. P., de Oliveira, F. D. S., & de Souza Teodoro, C. E. (2019). Antifungal activity of Cymbopogon citratus essential oil against Aspergillus flavus. Ciência e Natura, 41(20): 1-8. doi: 10.5902/2179460X36055
Munda, S., Dutta, S., Pandey, S. K., Sarma, N., & Lal, M. (2019). Antimicrobial activity of essential oils of medicinal and aromatic plants of the North east India: A biodiversity hot spot. Journal of Essential Oil Bearing Plants, 22(1), 105-119. doi: 10.1080/0972060X.2019.1601032
Pandey, A. K., Rai, M. K., & Acharya, D. (2003). Chemical composition and antimycotic activity of the essential oils of corn mint (Mentha arvensis) and lemon grass (Cymbopogon flexuosus) against human pathogenic fungi. Pharmaceutical Biology, 41(6), 421-425. doi: 10.1076/phbi.41.6.421.17825
Rojek, K., Serefko, A., Poleszak, E., Szopa, A., Wróbel, A., Guz, M., ... & Skalicka-Woźniak, K. (2021). Neurobehavioral properties of Cymbopogon essential oils and its components. Phytochemistry Reviews, 1-12. doi: 10.1007/s11101-020-09734-0
Simões, C.M.O. E Spitzer, V. Óleos Voláteis. In: Simões, C.M.O.; Schenkel, E.P.; Gosmann,G.; Mello, J.C.P.; Mentz, L.A.; Petrovick, P.R. Farmacognosia: Da Planta ao Medicamento. 5 ed. Porto Alegre: Editora da UFRGS, 2003. cap. 18, p. 467-495.
Tatsadjieu, N. L., Yaouba, A., Nukenine, E. N., Ngassoum, M. B., & Mbofung, C. M. F. (2010). Comparative study of the simultaneous action of three essential oils on Aspergillus flavus and Sitophilus zeamais Motsch. Food control, 21(2), 186-190. doi: 10.1016/j.foodcont.2009.05.004
Tian, F., Lee, S. Y., Woo, S. Y., Choi, H. Y., Heo, S., Nah, G., & Chun, H. S. (2021). Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production. Scientific reports, 11(1), 1-12. doi: 10.1038/s41598-021-82488-7
Xu, D., Wei, M., Peng, S., Mo, H., Huang, L., Yao, L., & Hu, L. (2021). Cuminaldehyde in cumin essential oils prevents the growth and aflatoxin B1 biosynthesis of Aspergillus flavus in peanuts. Food Control, 125(July) 107985. doi: 10.1016/j.foodcont.2021.107985
Zahra, A. A., Hartati, R., & Fidrianny, I. (2020). Review of the Chemical Properties, Pharmacological Properties, and Development Studies of Cymbopogon sp. Biointerface Research in Applied Chemistry, 11(3), 10341-10350. doi: 10.33263/BRIAC113.1034110350
Zhang, H., Chen, F., Wang, X., & Yao, H. Y. (2006). Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Research International, 39(8), 833-839. doi: 10.1016/j.foodres.2006.03.007
Adams, R. P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry (Vol. 456). Carol Stream, IL: Allured publishing corporation.
Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils–a review. Food and chemical toxicology, 46(2), 446-475. doi: 10.1016/j.fct.2007.09.106
Chao, S. C., Young, D. G., & Oberg, C. J. (2000). Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of essential oil research, 12(5), 639-649. doi: 10.1080/10412905.2000.9712177
Choi, H. S. (2003). Character impact odorants of Citrus Hallabong [(C. unshiu Marcov× C. sinensis Osbeck)× C. reticulata Blanco] cold-pressed peel oil. Journal of agricultural and food chemistry, 51(9), 2687-2692. doi: 10.1021/jf021069o
Billerbeck, V. G., Roques, C. G., Bessière, J. M., Fonvieille, J. L., & Dargent, R. (2001). Effects of Cymbopogon nardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger. Canadian journal of microbiology, 47(1), 9-17. doi: 10.1139/w00-117
Morais, L. A. S., Ramos, N. P., Gonçalves, G. G., Bettiol, W., & Chaves, F. C. M. (2008). Atividade antifúngica de óleos essenciais em sementes de feijão cv. Carioquinha. In Embrapa Amazônia Ocidental-Artigo em anais de congresso (ALICE). In: Congresso Brasileiro de Olericultura, 48., 2008, Maringá. Resumos... Maringá: Associação Brasileira de Horticultura, 2008. p. S6261-S6266
Dellavalle, P. D., Cabrera, A., Alem, D., Larrañaga, P., Ferreira, F., & Rizza, M. D. (2011). Antifungal activity of medicinal plant extracts against phytopathogenic fungus. Alternaria spp. Chilean journal of agricultural research, 71(2), 231-239. doi: 10.4067/S0718-58392011000200008
Ferreira, D. F. (2014). Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ciência e agrotecnologia, 38(2), 109-112. doi: 10.1590/S1413-70542014000200001.
Han, X., & Parker, T. L. (2017). Lemongrass (Cymbopogon flexuosus) essential oil demonstrated anti-inflammatory effect in pre-inflamed human dermal fibroblasts. Biochimie open, 4, 107-111. doi: 10.1016/j.biopen.2017.03.004
Lanças, F. M. (2009). A Cromatografia Líquida Moderna e a Espectrometria de Massas: finalmente “compatíveis”. Scientia chromatographica, 1(2), 35-61.
Lee, L. T., Garcia, S. A., Martinazzo, A. P., & Teodoro, C. E. S. (2020). Fungitoxity and chemical composition of rosemary essential oil (Rosmarinus officinalis) on Aspergillus flavus. Research, Society and Development, 9(8), e202985628. doi: 10.33448/rsd-v9i8.5628
Liu, Q., Li, X., Wu, R., Xiao, X., & Xing, F. (2021). Development of an on-spot and rapid recombinase polymerase amplification assay for Aspergillus flavus detection in grains. Food Control, 125, 107957 doi: 10.1016/j.foodcont.2021.107957
López-Malo, A., Alzamora, S. M., & Palou, E. (2002). Aspergillus flavus dose–response curves to selected natural and synthetic antimicrobials. International journal of food microbiology, 73(2-3), 213-218. doi: 10.1016/S0168-1605(01)00639-0
Lorenzi, H. (2008). Plantas daninhas do Brasil: terrestres, aquáticas, parasitas e tóxicas.
Luo, M., Jiang, L. K., Huang, Y. X., Xiao, M., Li, B., & Zou, G. L. (2004). Effects of citral on Aspergillus flavus spores by quasi-elastic light scattering and multiplex microanalysis techniques. Acta Biochimica et Biophysica Sinica, 36(4), 277-283. doi: 10.1093/abbs/36.4.277
Martinazzo, A. P., de Oliveira, F. D. S., & de Souza Teodoro, C. E. (2019). Antifungal activity of Cymbopogon citratus essential oil against Aspergillus flavus. Ciência e Natura, 41(20): 1-8. doi: 10.5902/2179460X36055
Munda, S., Dutta, S., Pandey, S. K., Sarma, N., & Lal, M. (2019). Antimicrobial activity of essential oils of medicinal and aromatic plants of the North east India: A biodiversity hot spot. Journal of Essential Oil Bearing Plants, 22(1), 105-119. doi: 10.1080/0972060X.2019.1601032
Pandey, A. K., Rai, M. K., & Acharya, D. (2003). Chemical composition and antimycotic activity of the essential oils of corn mint (Mentha arvensis) and lemon grass (Cymbopogon flexuosus) against human pathogenic fungi. Pharmaceutical Biology, 41(6), 421-425. doi: 10.1076/phbi.41.6.421.17825
Rojek, K., Serefko, A., Poleszak, E., Szopa, A., Wróbel, A., Guz, M., ... & Skalicka-Woźniak, K. (2021). Neurobehavioral properties of Cymbopogon essential oils and its components. Phytochemistry Reviews, 1-12. doi: 10.1007/s11101-020-09734-0
Simões, C.M.O. E Spitzer, V. Óleos Voláteis. In: Simões, C.M.O.; Schenkel, E.P.; Gosmann,G.; Mello, J.C.P.; Mentz, L.A.; Petrovick, P.R. Farmacognosia: Da Planta ao Medicamento. 5 ed. Porto Alegre: Editora da UFRGS, 2003. cap. 18, p. 467-495.
Tatsadjieu, N. L., Yaouba, A., Nukenine, E. N., Ngassoum, M. B., & Mbofung, C. M. F. (2010). Comparative study of the simultaneous action of three essential oils on Aspergillus flavus and Sitophilus zeamais Motsch. Food control, 21(2), 186-190. doi: 10.1016/j.foodcont.2009.05.004
Tian, F., Lee, S. Y., Woo, S. Y., Choi, H. Y., Heo, S., Nah, G., & Chun, H. S. (2021). Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production. Scientific reports, 11(1), 1-12. doi: 10.1038/s41598-021-82488-7
Xu, D., Wei, M., Peng, S., Mo, H., Huang, L., Yao, L., & Hu, L. (2021). Cuminaldehyde in cumin essential oils prevents the growth and aflatoxin B1 biosynthesis of Aspergillus flavus in peanuts. Food Control, 125(July) 107985. doi: 10.1016/j.foodcont.2021.107985
Zahra, A. A., Hartati, R., & Fidrianny, I. (2020). Review of the Chemical Properties, Pharmacological Properties, and Development Studies of Cymbopogon sp. Biointerface Research in Applied Chemistry, 11(3), 10341-10350. doi: 10.33263/BRIAC113.1034110350
Zhang, H., Chen, F., Wang, X., & Yao, H. Y. (2006). Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Research International, 39(8), 833-839. doi: 10.1016/j.foodres.2006.03.007
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Publicado
2021-09-30
Edição
Seção
Microbiologia
